CM Buckley, VL Heath, A Gueho, C Bosmani, P Knobloch, P Sikakana, N Personnic, SK Dove, RH Michell, R Meier, H Hilbi, T Soldati, RH Insalland JS King. PIKfyve/Fab1 is required for efficient V-ATPase and hydrolase delivery to phagosomes, phagosomal killing, and restriction of Legionella infection. bioRxiv 2018, 343301

AT Lopez-Jimenez, EC Munoz, F Leuba, L Gerstenmaier, M Hagedorn, JS Kingand T Soldati. ESCRT and autophagy cooperate to repair ESX-1-dependent damage to the Mycobacterium-containing vacuole. bioRxi 2018. 334755

RA Watkins, A Andrews, C Wynn, C Barisch, JS Kingand SA Johnston† .Cryptococcus neoformans escape from Dictyostelium amoeba by both WASH-mediated constitutive exocytosis and vomocytosis. Frontiers in Cellular and Infection Microbiology. 2018 doi: 10.3389/fcimb.2018.00108


RA Watkins, JS King, SA Johnston. Nutritional Requirements and Their Importance for Virulence of Pathogenic Cryptococcus Species. Microorganisms 5 (4), 65

Dominguez-Martin E, Cardenal-Munoz E, King JS, Soldati T, Coria R and Escalante R. Methods to Monitor and Quantify Autophagy in the Social Amoeba Dictyostelium discoideum. Cells 2017 6, 18; doi:10.3390/cells6030018

Buckley CM and King JS. Drinking problems: Mechanisms of macropinosome formation and maturation. FEBS Journal 2017 May 24. doi: 10.1111/febs.14115.

Cardenal-Muñoz E, Arafah S, López-Jiménez AT,Kicka S, Falaise A, Bach F, King JS, Hagedorn M, Soldati T. Mycobacterium marinum antagonistically induces an autophagic response while repressing the autophagic flux in a TORC1- and ESX-1-dependent manner. PLoS Pathogens. 13(4): e1006344.

Thomason P, King JS, Insall RH. Mroh1, a lysosomal regulator localised by WASH-generated actin. J. Cell Sci. May 15;130(10):1785-1795

Mesquita A, Cardenal-Muñoz E, Dominguez E, Muñoz-Braceras S, Nuñez-Corcuera B, Phillips BA, Tábara LC, Xiong Q, Coria R, Eichinger L, Golstein P, King JS, Soldati T, Vincent O, Escalante R. Autophagy in Dictyostelium: Mechanisms, regulation and disease in a simple biomedical model. Autophagy. 2016 Oct 7:1-17.


Buckley CM*, Gopaldass N*, Bosmani C, Johnston SA, Soldati T, Insall RH, King JS. WASH drives early recycling from macropinosomes and phagosomes to maintain surface phagocytic receptors. Proceedings of the National Academy of Sciences, USA. 2016 Oct 4;113(40):E5906-E5915

Klionsky DJ. et al. Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy (3rd Edition) Autophagy. 2016;12(1):1-222.


Gerstenmaier L, Pilla R, Herrmann L, Herrmann H, Prado M, Villafano GJ, Kolonko M, Reimer R, Soldati T, King JS, Hagedorn M. The autophagic machinery ensures nonlytic transmission of mycobacteria. Proceedings of the National Academy of Sciences USA, 2015. 112 (7), E687-E692

Urushihara H, Kuwayama H, Fukuhara K, Itoh T, Kagoshima H, Shin-I T, Toyoda A, Ohishi K, Taniguchi T, Noguchi H, Kuroki Y, Hata T, Uchi K, Mohri K, King JS, Insall RH, Kohara Y, Fujiyama A. Comparative genome and transcriptome analyses of the social amoeba Acytostelium subglobosum that accomplishes multicellular development without germ-soma differentiation. BMC Genomics 2015. 16 (1) 80.


Calvo-Garrido J*, King JS*, Munoz-Braceras S, Escalante R. Vmp1 regulates PtdIns3P signaling during autophagosome formation in Dictyostelium discoideum. Traffic 2014. 15, 1235-1246.


King JS, Gueho A, Hagedorn M, Gopaldass N, Leuba F, Soldati T and Insall RS. WASH is required for lysosomal recycling and efficient autophagic and phagocytic digestion. Mol. Biol. Cell. 2013. 24 (17), 2714-2726

Davidson AJ*, King, JS*, RH Insall. The use of streptavidin conjugates as immunoblot loading controls and mitochondrial markers for use with Dictyostelium discoideum. BioTechniques 2013. 55, 39-41

Park L, Thomason PS, Zech T, King JS, Ura S, Carnell M, Machesky LM and Insall RH. Cyclical action of the WASH complex: FAM21 and capping protein drive WASH recycling, not initial recruitment.  Dev. Cell. 2013 Jan 28;24(2):169-81


King, JS. Mechanical stress meets autophagy: potential implications for physiology and pathology. Trends Mol. Med. 2012. 18(10) pp. 583 – 588

Veltman DM, King JS, Machesky LM and Insall RH. SCAR knockouts in Dictyostelium: WASP assumes SCAR’s position and upstream regulators in pseudopods. J. Cell Biol. 2012; 198(4): 501-508

King JS. Autophagy across the eukaryotes: Is S. cerevisiae the odd one out?.  Autophagy. 2012 Jul 1;8(7)

Klionsky DJ. et al. Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy, 2012 Apr 1;8(4).


King JS, Veltman DM and Insall RH. The induction of autophagy by mechanical stress. Autophagy. 2011 Dec 1;7(12).


King JS, Veltman DM, Georgiou M, Baum B, Insall RH. SCAR/WAVE is activated at mitosis and drives myosin-independent cytokinesis. J. Cell Sci. 2010 Jul 1;123(Pt 13): 2246-55.

King JS, Keim M, Teo R, Weening KE, Kapur M, McQuillan K, Ryves J, Rogers B, Dalton E, Williams RSB and Harwood AJ. Genetic control of lithium sensitivity and regulation of inositol biosynthetic genes. PLOS One, 2010 Jun 17;5(6):e11151.


Teo R, King J, Dalton E, Ryves J, Williams RS, Harwood AJ. PtdIns (3, 4, 5) P3 and inositol depletion as a cellular target of mood stabilizers. Biochem Soc Trans. 2009 Oct;37(Pt 5):1110-4.

King JS, Insall RH. Chemotaxis: finding the way forward with Dictyostelium. Trends Cell Biol. 2009 Oct;19(10):523-30.

King JS*, Teo R*, Ryves J, Reddy JV, Peters O, Orabi B, Hoeller O, Williams RS, Harwood AJ. The mood stabiliser lithium suppresses PIP3 signalling in Dictyostelium and human cells. Dis Model Mech. 2009 May-Jun;2(5-6):306-12.


Cho J, King JS, Qian X, Harwood AJ, Shears SB. Dephosphorylation of 2, 3-bisphosphoglycerate by MIPP expands the regulatory capacity of the Rapoport–Luebering glycolytic shunt. Proc Natl Acad Sci U S A. 2008 Apr 22;105(16):5998-6003.


Kortholt A*, King JS*, Keizer-Gunnink I, Harwood AJ, Van Haastert PJ. Phospholipase C regulation of phosphatidylinositol 3, 4, 5-trisphosphate-mediated chemotaxis. Mol Biol Cell. 2007 Dec;18(12):4772-9.

Zaki M*, King J*, Futterer K, Insall RH. Replacement of the essential Dictyostelium Arp2 gene by its Entamoeba homologue using parasexual genetics. BMC Genet. 2007 Jun 6;8:28.


King J and Insall R. Parasexual genetics using axenic cells. Methods Mol Biol. 2006;346:125-35.


King J and Insall RH. Parasexual genetics of Dictyostelium gene disruptions: identification of a ras pathway using diploids. BMC Genet. 2003 Jul 10;4:12.

University of Sheffield